Solid-Phase Cytometry: The Core of Redberry Technology

Solid-Phase Cytometry:

The Core of Redberry Technology

Solid-Phase Cytometry (SPC) is a cutting-edge technique that enables the direct detection and quantification on a membrane. Unlike traditional cytometry methods, which rely on cells flowing through a detector, SPC combines imaging, fluorescence staining, and the immobilization of cells on a track-etched membrane to deliver ultra-sensitive and highly selective microbial detection.

To date, it remains the only technology capable of detecting as few as a single cell in filterable samples.

Earlier generations of SPC, while demonstrating high sensitivity, were also labor-intensive, requiring multiple manual handling steps and complex workflows. These limitations not only reduced throughput and increased the risk of cross-contamination, but also hindered widespread adoption, particularly in routine or high-throughput settings.

At Redberry, we’ve reimagined SPC by removing the barriers that once limited its use. Through the integration of innovative optics, advanced viability staining chemistry, and powerful AI-based image analysis within a fully automated system, we’ve developed a next-generation platform capable of reliably detecting single cells—even in large and complex samples. With Redberry, SPC becomes faster, simpler, and truly accessible—ready for real-world applications at scale.

To date, it remains the only technology capable of detecting as few as a single cell in filterable samples.

Earlier generations of SPC, while demonstrating high sensitivity, were also labor-intensive, requiring multiple manual handling steps and complex workflows. These limitations not only reduced throughput and increased the risk of cross-contamination, but also hindered widespread adoption, particularly in routine or high-throughput settings.

At Redberry, we’ve reimagined Solid-Phase Cytometry by removing the barriers that once limited its use. Through the integration of innovative optics, advanced viability staining chemistry, and powerful AI-based image analysis within a fully automated system, we’ve developed a next-generation platform capable of reliably detecting single cells—even in large and complex samples. With Redberry, SPC becomes faster, simpler, and truly accessible—ready for real-world applications at scale.

To date, it remains the only technology capable of detecting as few as a single cell in filterable samples.

Earlier generations of SPC, while demonstrating high sensitivity, were also labor-intensive, requiring multiple manual handling steps and complex workflows. These limitations not only reduced throughput and increased the risk of cross-contamination, but also hindered widespread adoption, particularly in routine or high-throughput settings.

At Redberry, we’ve reimagined Solid-Phase Cytometry by removing the barriers that once limited its use. Through the integration of innovative optics, advanced viability staining chemistry, and powerful AI-based image analysis within a fully automated system, we’ve developed a next-generation platform capable of reliably detecting single cells—even in large and complex samples. With Redberry, SPC becomes faster, simpler, and truly accessible—ready for real-world applications at scale.

What Makes Redberry’s SPC Unique?

What Makes Redberry’s SPC Unique?

At the heart of Redberry technology lies our patented approach to SPC, built around three key innovations:

High-Resolution Imaging

Red One™ uses a high-resolution CMOS camera to capture detailed images of immobilized microorganisms. Powerful LED illumination ensures uniform and intense lighting, revealing even faint fluorescent signals with precision.

High-Resolution Imaging

Red One™ uses a high-resolution CMOS camera to capture detailed images of immobilized microorganisms. Powerful LED illumination ensures uniform and intense lighting, revealing even faint fluorescent signals with precision.

High-Resolution Imaging

Red One™ uses a high-resolution CMOS camera to capture detailed images of immobilized microorganisms. Powerful LED illumination ensures uniform and intense lighting, revealing even faint fluorescent signals with precision.

Viability-Based Fluorescent Staining

We use a proprietary fluorescein derivative that targets esterase activity—a marker of cellular viability. This ensures only live cells emit a measurable fluorescent signal, providing both sensitivity and selectivity in detection.

Viability-Based Fluorescent Staining

We use a proprietary fluorescein derivative that targets esterase activity—a marker of cellular viability. This ensures only live cells emit a measurable fluorescent signal, providing both sensitivity and selectivity in detection.

Viability-Based Fluorescent Staining

We use a proprietary fluorescein derivative that targets esterase activity—a marker of cellular viability. This ensures only live cells emit a measurable fluorescent signal, providing both sensitivity and selectivity in detection.

Time-Resolved Detection (Staining Kinetics)

Unlike conventional methods, Redberry monitors the fluorescence evolution over time, a process known as staining kinetics. Images are captured before, during, and after staining, allowing the system to distinguish viable cells from inert or autofluorescent particles through dynamic signal analysis.

Time-Resolved Detection (Staining Kinetics)

Unlike conventional methods, Redberry monitors the fluorescence evolution over time, a process known as staining kinetics. Images are captured before, during, and after staining, allowing the system to distinguish viable cells from inert or autofluorescent particles through dynamic signal analysis.

Time-Resolved Detection (Staining Kinetics)

Unlike conventional methods, Redberry monitors the fluorescence evolution over time, a process known as staining kinetics. Images are captured before, during, and after staining, allowing the system to distinguish viable cells from inert or autofluorescent particles through dynamic signal analysis.

By leveraging staining kinetics, Redberry SPC eliminates over 99% of non-specific signals from inert or autofluorescent particles. This significantly improves the accuracy of microbial counts, especially in complex, particulate-rich or cell-based samples, where conventional techniques often fail

Staining Kinetics